epidemiologyЭпидемиология и ВакцинопрофилактикаEpidemiology and Vaccinal Prevention2073-30462619-0494«Numicom» LLC10.31631/2073-3046-2020-19-4-38-47epidemiology-1053Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESМолекулярно-генетическое типирование Acinetobacter baumannii, выделенных из гемокультуры больных опухолями системы крови, методом ПЦР случайных полиморфных фрагментов ДНКGenotyping by Random Amplified Polymorphic DNA Assay of Acinetobacter baumannii Isolated from Blood Culture of Patients with Hematological Malignancieshttps://orcid.org/0000-0002-1127-3333ХрульноваС. А.KhrulnovaS. A.

Светлана Алексеевна Хрульнова – старший научный сотрудник лаборатории клинической бактериологии, микологии и антибиотической терапии

+7 (495)-614-92-72

Svetlana A. Khrulnova  – Senior researcher, Laboratory of clinical bacteriology, mycology, and antibiotic treatment 

+7 (495)-614-92-72

khrulnovas@mail.ruhttps://orcid.org/0000-0003-3919-1150ФедороваА. В.FedorovaA. V.

Анастасия Владимировна Федорова – старший научный сотрудник лаборатории клинической бактериологии, микологии и  антибиотической терапии 

+7 (495)-614-92-72 

Anastasija V. Fedorova – Senior researcher, Laboratory of clinical bacteriology, mycology, and antibiotic treatment 

+7 (495)- 614-92-72

mirnas19@yandex.ruhttps://orcid.org/0000-0001-9308-9259ФроловаИ. Н.FrolovaI. N.

Ирина Николаевна Фролова – научный сотрудник лаборатории клинической бактериологии, микологии и  антибиотической терапии 

+7 (495)-614-92-72

Irina N. Frolova – Researcher, Laboratory of clinical bacteriology, mycology, and antibiotic treatment 

+7 (495)-614-92-72

Frolova.i@blood.ruhttps://orcid.org/0000-0001-5973-5763КлясоваГ. А.KlyasovaG. A.

Галина Александровна Клясова  – заведующая лабораторией клинической бактериологии, микологии и  антибиотической терапии 

+7 (495)-614-92-72

Galina A. Klyasova – Head of Laboratory of clinical bacteriology, mycology, and antibiotic treatment 

+7 (495)-614-92-72

klyasova.g@blood.ruФГБУ «Национальный медицинский исследовательский центр гематологии» Минздрава РоссииРоссияNational Research Center for HematologyRussian Federation2020110920201943847Copyright © Хрульнова С.А., Федорова А.В., Фролова И.Н., Клясова Г.А., 20202020Хрульнова С.А., Федорова А.В., Фролова И.Н., Клясова Г.А.Khrulnova S.A., Fedorova A.V., Frolova I.N., Klyasova G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.epidemvac.ru/jour/article/view/1053Acinetobacter baumanniiгемокультураопухоли системы кровиRAPD-ПЦРгенотипированиеустойчивость к карбапенемамOXA-карбапенемазыAcinetobacter baumanniihematological malignanciesblood culturegenotypingRAPD-PCRcarbapenem resistanceOXA-carbapenemasesReferencesGedik H., Simşek F., Kantürk A., et al. Bloodstream infections in patients with hematological malignancies: which is more fatal - cancer or resistant pathogens? // Ther Clin Risk Manag. 2014. Vol. 10, P. 743-752. https://doi.org/10.2147/tcrm.s68450.Gedik H, Simşek F, Kantürk A, et al. Bloodstream infections in patients with hematological malignancies: which is more fatal - cancer or resistant pathogens? Ther Clin Risk Manag. 2014;10:743–752. https://doi.org/10.2147/tcrm.s68450.Wang X., Zhang L., Sun A., et al. Acinetobacter baumannii bacteraemia in patients with haematological malignancy: a multicenter retrospective study from the Infection Working Party of Jiangsu Society of Hematology. // Eur J Clin Microbiol Infect Dis. 2017. Vol. 36, N7. P. 1073–1081. https://doi.org/10.1007/s10096-016-2895-2.Wang X, Zhang L, Sun A, et al. Acinetobacter baumannii bacteraemia in patients with haematological malignancy: a multicenter retrospective study from the Infection Working Party of Jiangsu Society of Hematology. Eur J Clin Microbiol Infect Dis. 2017;36(7):1073–1081. https://doi.org/10.1007/s10096-016-2895-2.Клясова Г. А., Сперанская Л. Л., Миронова А. В., и др. Возбудители сепсиса у иммунокомпрометированных больных: структура и проблемы антибиотикорезистентности (результаты многоцентрового исследования) // Гематология и трансфузиология. 2007. Т. 52, №1. С. 13–18.Klyasova GА, Speranskaya LL, Mironova АV, et al. The pathogens causing sepsis in immunocompromized patients: structure and problems of antibiotic resistance. Results of a multi-center cooperative study. Gemаtologiya i trаnsfuziologiya. 2007;52(1):3–18 (In Russ).Клясова Г. А. Охмат В. А. Антимикробная терапия. Под редакцией Савченко В.Г. В кн: Алгоритмы диагностики и протоколы лечения заболеваний системы крови. М.: Практика; 2018. С. 1067–1113.Klyasova GА, Okhmat VА. Antimicrobial therapy. In: Savchenko VG, ed. Algorithms of diagnosing and protocols of treatment of blood system diseases. Moscow: Praktika; 2018:1069–1113 (In Russ).Ballouz T., Aridi J., Afif C., et al. Risk factors, clinical presentation, and outcome of Acinetobacter baumannii bacteremia. // Front Cell Infect Microbiol. 2017. N7. P. 156. https://doi.org/10.3389/fcimb.2017.00156.Ballouz T, Aridi J, Afif C, et al. Risk factors, clinical presentation, and outcome of Acinetobacter baumannii bacteremia. Front Cell Infect Microbiol. 2017;7:156. https://doi.org/10.3389/fcimb.2017.00156.Freire M.P., de Oliveira Garcia D., Garcia C.P., et al. Bloodstream infection caused by extensively drug-resistant Acinetobacter baumannii in cancer patients: high mortality associated with delayed treatment rather than with the degree of neutropenia // Clin Microbiol Infect. 2016. N 22. P. 352–358. https://doi.org/10.1016/j.cmi.2015.12.010.Freire MP, de Oliveira Garcia D, Garcia C.P., et al. Bloodstream infection caused by extensively drug-resistant Acinetobacter baumannii in cancer patients: high mortality associated with delayed treatment rather than with the degree of neutropenia. Clin Microbiol Infect. 2016;22:352–358. https://doi.org/10.1016/j.cmi.2015.12.010.Shargian-Alon L., Gafter-Gvili A., Ben-Zvi H., et al. Risk factors for mortality due to Acinetobacter baumannii bacteremia in patients with hematological malignancies - a retrospective study. // Leuk Lymphoma. 2019. Vol. 60, N11. P. 2787–2792. https://doi.org/10.1080/10428194.2019.1599113.Shargian-Alon L, Gafter-Gvili A, Ben-Zvi H, et al. Risk factors for mortality due to Acinetobacter baumannii bacteremia in patients with hematological malignancies - a retrospective study. Leuk Lymphoma. 2019;60(11):2787–2792. https://doi.org/10.1080/10428194.2019.1599113.Lee C.R., Lee J.H., Park M., et al. Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options. // Front Cell Infect Microbiol. 2017. N7. P. 55. https://doi.org/10.3389/fcimb.2017.00055.Lee CR, Lee JH, Park M, et al. Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options. Front Cell Infect Microbiol. 2017;7:55. https://doi.org/10.3389/fcimb.2017.00055.Хрульнова С. А., Коробова А. Г., Федорова А. В. и др. Детекция генов приобретенных карбапенемаз у изолятов Acinetobacter baumannii, выделенных из гемокультуры больных опухолями системы крови. // Клиническая микробиология и антимикробная химиотерапия. 2019. Т. 21, № 1. С. 50–55. https://doi.org/10.36488/cmac.2019.1.56-60.Khrulnova SA, Korobova AG, Fyodorova AV, et al. Detection of acquired carbapenemase genes among Acinetobacter baumannii isolated from blood culture in patients with hematological malignancies. Clinical Microbiology and Antimicrobial Chemotherapy. 2019;21(1):56–60. (In Russ). https://doi.org/10.36488/cmac.2019.1.56-60.Chung D.R., Song J.H., Kim S.H., et al. High prevalence of multidrug-resistant nonfermenters in hospital-acquired pneumonia in Asia. // Am J Respir Crit Care Med. 2011. N184. P. 1409–1417. https://doi.org/10.1164/rccm.201102-0349oc.Chung DR, Song JH, Kim SH, et al. High prevalence of multidrug-resistant nonfermenters in hospital-acquired pneumonia in Asia. Am J Respir Crit Care Med. 2011;184:1409–1417. https://doi.org/10.1164/rccm.201102-0349oc.Шек Е. А., Сухорукова М. В., Эйдельштейн М. В. и др. Антибиотикорезистентность, продукция карбапенемаз и генотипы нозокомиальных штаммов Acinetobacter spp. в стационарах России: результаты многоцентрового эпидемиологического исследования «МАРАФОН 2015–2016». // Клиническая микробиология и антимикробная химиотерапия. 2019. Т.21, №2. С. 171–180. https://doi.org/10.36488/cmac.2019.2.171-180.Shek EA, Sukhorukova MV, Edelstein MV, et al. Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study «MARATHON 2015–2016». Clinical Microbiology and Antimicrobial Chemotherapy. 2019;21(2):171–80 (In Russ). https://doi.org/10.36488/cmac.2019.2.171-180.Strateva T., Sirakov I., Stoeva T., et al. Carbapenem-resistant Acinetobacter baumannii: Current status of the problem in four Bulgarian university hospitals (2014–2016). // J Glob Antimicrob Resist. 2019. N16. P. 266-273. https://doi.org/10.1016/j.jgar.2018.10.027.Strateva T, Sirakov I, Stoeva T, et al. Carbapenem-resistant Acinetobacter baumannii: Current status of the problem in four Bulgarian university hospitals (2014-2016). J Glob Antimicrob Resist. 2019;16:266–273. https://doi.org/10.1016/j.jgar.2018.10.027.Evans B.A., Amyes S.G. OXA β-lactamases. // Clin Microbiol Rev. 2014. N27. P. 241–263. https://doi.org/10.1128/cmr.00117-13.Evans BA, Amyes SG. OXA β-lactamases. Clin Microbiol Rev. 2014;27:241–263. https://doi.org/10.1128/cmr.00117-13.Munoz-Price L.S., Namias N., Cleary T., et al. Acinetobacter baumannii: association between environmental contamination of patient rooms and occupant status. // Infect Control Hosp Epidemiol. 2013. Vol. 34, N5. P. 517–20. https://doi.org/10.1086/670209.Munoz-Price LS, Namias N, Cleary T, et al. Acinetobacter baumannii: association between environmental contamination of patient rooms and occupant status. Infect Control Hosp Epidemiol. 2013;34(5):517–20. https://doi.org/10.1086/670209.Morgan D.J., Liang S.Y., Smith C.L., et al. Frequent multidrug-resistant Acinetobacter baumannii contamination of gloves, gowns, and hands of healthcare workers. // Infect Control Hosp Epidemiol. 2010. Vol. 31, N7. P. 716–21. https://doi.org/10.1086/653201.Morgan DJ, Liang SY, Smith CL, et al. Frequent multidrug-resistant Acinetobacter baumannii contamination of gloves, gowns, and hands of healthcare workers. Infect Control Hosp Epidemiol. 2010;31(7):716–21. https://doi.org/10.1086/653201.Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Seventh Informational Supplement. CLSI document M100-S28. Wayne, PA: Clinical and Laboratory Standarts Institute; 2018.Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Seventh Informational Supplement. CLSI document M100-S28. Wayne, PA: Clinical and Laboratory Standarts Institute; 2018.Dahdouh E., Gómez-Gil R., Sanz S., et al. A novel mutation in pmrB mediates colistin resistance during therapy of Acinetobacter baumannii // Int J Antimicrob Agents. 2017. Vol. 49, N6. P. 727–733. https://doi.org/10.1016/j.ijantimicag.2017.01.031.Dahdouh E, Gómez-Gil R, Sanz S, et al. A novel mutation in pmrB mediates colistin resistance during therapy of Acinetobacter baumannii. Int J Antimicrob Agents. 2017;49(6):727–733. https://doi.org/10.1016/j.ijantimicag.2017.01.031.Heras J., Domínguez C., Mata E., et al. GelJ – a tool for analyzing DNA fingerprint gel images. BMC Bioinformatics. 2015;16:270. https://doi.org/10.1186/s12859-015-0703-0.Heras J, Domínguez C, Mata E, et al. GelJ – a tool for analyzing DNA fingerprint gel images. BMC Bioinformatics. 2015;16:270. https://doi.org/10.1186/s12859-015-0703-0Hunter PR. Reproducibility and indices of discriminatory power of microbial typing methods // J Clin Microbiol. 1990;28(9):1903–1905. https://doi.org/10.1128/jcm.28.9.1903-1905.1990.Hunter PR. Reproducibility and indices of discriminatory power of microbial typing methods. J Clin Microbiol. 1990;28(9):1903–1905. https://doi.org/10.1128/jcm.28.9.1903-1905.1990.Гординская Н. А., Сабирова Е. В., Абрамова Н.В. и др. Антибиотикочувствительность и молекулярные механизмы резистентности Acinetobacter baumanii, возбудителей раневой ожоговой инфекции. // Медицинский альманах. 2015. Т. 5, № 40. С. 99–101.Gordinskaya NA, Sabirova EV, Abramova NV et al. Antibiotics sensitivity and molecular mechanisms of resistance of Acinetobacter baumanii, infectious agents of burn-wound infection. Medicinskij almanah. 2015; 5(40):99–101 (In Russ).Mayanskiy N., Chebotar I., Alyabieva N., et al. Emergence of the Uncommon Clone ST944/ST78 Carrying blaOXA-40-like and blaCTX-M-like Genes Among Carbapenem-Nonsusceptible Acinetobacter baumannii in Moscow, Russia. // Microb Drug Resist. 2017. Vol. 23, N7. P. 864–870. https://doi.org/10.1089/mdr.2016.0302.Mayanskiy N, Chebotar I, Alyabieva N, et al. Emergence of the Uncommon Clone ST944/ST78 Carrying blaOXA-40-like and blaCTX-M-like Genes Among Carbapenem-Nonsusceptible Acinetobacter baumannii in Moscow, Russia. Microb Drug Resist. 2017;23(7):864–870. https://doi.org/10.1089/mdr.2016.0302.Alsultan A.A., Aboulmagd E., Evans B.A., et al. Clonal diversity of Acinetobacter baumannii from diabetic patients in Saudi Arabian hospitals. // J Med Microbiol. 2014. Vol. 63, N11. P. 1460–1466. https://doi.org/10.1099/jmm.0.079640-0.Alsultan AA, Aboulmagd E, Evans BA, et al. Clonal diversity of Acinetobacter baumannii from diabetic patients in Saudi Arabian hospitals. J Med Microbiol. 2014;63(11):1460–66. https://doi.org/10.1099/jmm.0.079640-0.

The authors declare that there are no conflicts of interest present.