epidemiologyЭпидемиология и ВакцинопрофилактикаEpidemiology and Vaccinal Prevention2073-30462619-0494«Numicom» LLC10.31631/2073-3046-2021-20-4-48-56epidemiology-1317Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESОбнаружение в Российской Федерации устойчивого к ципрофлоксацину негруппируемого штамма Neisseria meningitidis клонального комплекса ST-175The Emergence of a Ciprofloxacin-Resistant Non-Groupable Neisseria meningitidis Strain of the Clonal Complex ST-175 in the Russian Federationhttps://orcid.org/0000-0002-2714-1191КоролеваМ. А.KorolevaM. A.

Королева Мария Александровна, к. м. н., старший научный сотрудник лаборатории эпидемиологии менингококковой инфекции и гнойных бактериальных менингитов

Россия, 111123, Москва, ул. Новогиреевская, д. 3а

+7 (916) 363-82-48

Koroleva Maria А., Cand. Sci. (Med.), Senior Researcher, Laboratory for Epidemiology of Meningococcal Infection and Purulent Bacterial Meningitis

3a, st. Novogireevskaya, Moscow, 111123, Russia

+7 (916) 363-82-48

korolevamaria389@gmail.comГрицайМ. И.GritsayM. I.

Мария Игоревна Грицай – аспирант лаборатории эпидемиологии менингококковой инфекции и гнойных бактериальных менингитов

Москва

Maria I. Gritsay – Graduate Student, Laboratory for Epidemiology of Meningococcal Infection and Purulent Bacterial Meningitis

Moscow

maria-griz@mail.ruhttps://orcid.org/0000-0001-8207-9215МироновК. О.MironovK. O.

Константин Олегович Миронов – д. м. н., руководитель научной группы разработки новых методов выявления генетических полиморфизмов

Москва

Konstantin O. Mironov – Dr. Sci. (Med.), Head of scientific group for genetic polymorphism detection

Moscow

mironov@pcr.ruhttps://orcid.org/0000-0002-5646-538XМихайловаЮ. В.MikhailovaY. V.

Юлия Владимировна Михайлова – к. б. н., заведующий лабораторией молекулярных механизмов антибиотикорезистентности

Москва

Yuliya V. Mikhaylova – Cand. Sci. (Bio.), head of laboratory of molecular mechanisms of antibiotic resistance

Moscow

mihailova@cmd.suhttps://orcid.org/0000-0002-7409-077XШеленковА. А.ShelenkovA. A.

Андрей Александрович Шеленков – к. ф-м. н., старший научный сотрудник лаборатории молекулярных механизмов антибиотикорезистентности

Москва

Andrey A. Shelenkov – Cand. Sci. (FM.), senior researcher of Laboratory of molecular mechanisms of antibiotic resistance

Moscow

shelenkov@cmd.suhttps://orcid.org/0000-0003-0578-146XКоролеваИ. С.KorolevaI. S.

Ирина Станиславовна Королева – д. м. н., заведующая лабораторией эпидемиологии менингококковой инфекции и гнойных бактериальных менингитов

Москва

Irina S. Koroleva – Dr. Sci. (Med.), head of Laboratory for Epidemiology of Meningococcal Infection and Purulent Bacterial Meningitis

Moscow

irina-korol@yandex.ruФБУН «Центральный научно-исследовательский институт эпидемиологии» РоспотребнадзораРоссияCentral Research Institute of EpidemiologyRussian Federation2021030920212044856Copyright © Королева М.А., Грицай М.И., Миронов К.О., Михайлова Ю.В., Шеленков А.А., Королева И.С., 20212021Королева М.А., Грицай М.И., Миронов К.О., Михайлова Ю.В., Шеленков А.А., Королева И.С.Koroleva M.A., Gritsay M.I., Mironov K.O., Mikhailova Y.V., Shelenkov A.A., Koroleva I.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.epidemvac.ru/jour/article/view/1317Актуальность

Актуальность. Неинкапсулированные штаммы менингококка (NmNG) очень редко вызывают генерализованную форму менингококковой инфекции. Устойчивый к ципрофлоксацину новый штамм NmNG ST-175 cc175, который недавно стал причиной нескольких случаев инвазивной менингококковой инфекции в Европе, обнаружен в Российской Федерации.

Цель

Цель. Сопоставить новые российские штаммы NmNG ST-175 с уже охарактеризованными NmNG ST-175 и проанализировать генетические маркеры, ассоциированные с устойчивостью к антибиотикам

Материалы и методы

Материалы и методы. Нуклеотидные последовательности штаммов NmNG ST-175 размером более 2 млн пар оснований были экспортированы из базы данных PubMLST. Сравнение проводилось по 1605 локусам «основного генома» (core genome) с использованием опции «N. meningitidis cgMLST v1.0». Генетические взаимоотношения 127 штаммов NmNG ST-175, в том числе 8 российских носительских штаммов, были визуализированы с использованием программы SplitsTree (версия 4.16.2).

Результаты и обсуждение

Результаты и обсуждение. Из восьми российских штаммов шесть оказались в кластере с немецкими и шведским изолятами, вызвавшими инвазивную менингококковую инфекцию. Еще два российских штамма были наиболее близки к изолятам кластера, включающего носительские изоляты из Англии. Семь штаммов проявили резистентность к ципрофлоксацину и обладали аллелями gyrA-187 и gyrA-152, которые на основании филогенетического анализа аллелей относились к генетическим ветвям Neisseria cinerea и Nm.

Заключение

Заключение. Только белковые вакцины потенциально могли бы обеспечить защиту от NmNG ST-175. Перспективным представляется изучение антигенных характеристик российских штаммов менингококка, в том числе NmNG ST-175, для оценки потенциального охвата вакцинацией существующими белковыми вакцинами, возможности их регистрации на территории Российской Федерации, а также разработки отечественных вакцин.

Relevance

Relevance. Unencapsulated strains of meningococcus (NmNG) very rarely cause invasive meningococcal disease. A new ciprofloxacin-resistant strain NmNG ST-175 cc175, which has recently caused several cases of invasive meningococcal infection in Europe, has been discovered in the Russian Federation.

Aim

Aim. To compare the new Russian strains of NmNG ST-175 with the already characterized NmNG ST-175 and to analyze the genetic markers associated with antibiotic resistance.

Materials and methods

Materials and methods. The nucleotide sequences of NmNG ST-175 strains of more than 2 million base pairs were exported from the PubMLST database. Comparison was carried out for 1605 core genome loci using the N. meningitidis cgMLST v1.0 ". Genetic relationships of 127 NmNG ST-175 strains, including 8 Russian carrier strains, were visualized using the SplitsTree software (version 4.16.2).

Results

Results. Of the eight Russian strains, six found themselves in a cluster with German and Swedish isolates that caused invasive meningococcal infection. Two more Russian strains were closest to the isolates of the cluster, including carrier isolates from England. Seven strains showed resistance to ciprofloxacin and possessed the gyrA-187 and gyrA-152 alleles, which, based on the phylogenetic analysis of the alleles, belonged to the genetic branches of Neisseria cinerea and Nm.

Conclusion

Conclusion. Protein vaccines alone could potentially provide protection against ST-175 NmNG. It seems promising to study the antigenic characteristics of Russian Nm strains, including NmNG ST-175, to assess the potential vaccination coverage with existing protein vaccines, the possibility of their registration on the territory of the Russian Federation, as well as the development of domestic vaccines.

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The authors declare that there are no conflicts of interest present.