The effect of Locally Applied Bacillus safensis Strain 440-1 from Antarctic Soil on the Course of Experimental Wound Staphylococcal Infection

Relevance. Infections caused by methicillin-resistant strains of Staphylococcus aureus are an urgent problem of hospital epidemiology, and control measures involve the development of new antimicrobials. Probiotics based on bacterial strains isolated fro natural habitats are considered promising means of combating MRSA.

Aims. To test the possibility of using the Bacillus safensis 440-1 strain isolated from Antarctic soil. as an antibacterial agent for the treatment of experimental staphylococcal infection.

Purpose of the study – check the possibility of using the Bacillus safensis 440-1 strain, isolated from Antarctic soil, as a topical antibacterial agent for the treatment of experimental staphylococcal infection.

Material and methods. The potential virulence of Bacillus safensis strain 440-1 was studied on a mouse peritonitis model, and its activity against the methicillin-resistant Staphylococcus aureus SA776 test strain was studied on a localized wound infection model.

Results and discussion. Our study demonstrated the safety of the tested strain due intraperitoneal use. We found that the use of the studied strain reduces the staphylococci amount in wounds from the third day from the start of the study to the end of the observation period, without aggravating the course of the infection.

Conclusion. Bacillus safensis strain is a promising potential probiotic and it can be used as a part of complex antiseptic or disinfectant after additional studies aimed at developing an optimal mode of its use.

1. Tacconelli E., Carrara E., Savoldi A., et al. WHO Pathogens Priority List Working Group. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Dis. 2018;18(3):318–327. doi: 10.1016/S1473-3099(17)30753-3

2. Lai CC, Chen SY, Ko WC., et al. Increased antimicrobial resistance during the COVID-19 pandemic. Int J Antimicrob Agents. 2021;57(4):106324. doi: 10.1016/j.ijantimicag.2021.106324

3. Jalalifar S, Mirzaei R, Motallebirad T, et al. The Emerging Role of Probiotics and their Derivatives against Biofilm-Producing MRSA: A Scoping Review. Biomed Res Int. 2022;2022:4959487. doi: 10.1155/2022/4959487

4. Aslanov BI, Goncharov AE, Azarov DV, et al. Bioprospecting of potential producers of new antimicrobial compounds in terrestrial ecosystems of Antarctica. Preventive and clinical medicine. 2022; 4(85):20–24 (In Russ). doi: 10.47843/2074-9120_2022_4_20

5. European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (ETS No. 123). Available at: https://rm.coe.int/168007a67b. Accessed: 12 October 2023.

6. Federal Service for Supervision of Consumer Rights Protection and Human Welfare. Methodical instructions. MUK 4.2.2602-10. System of pre-registration preclinical study of drug safety. Selection, testing and storage of production strains used in the production of probiotics. Available at: https://www.rospotrebnadzor.ru/bitrix/redirect.php?event1=file&event2=download&event3=muk-4.2.2602_10.doc&goto=/upload/iblock/35b/muk-4.2.2602_10.doc. Accessed: 12 October 2023 (In Russ).

7. Goncharov AE, Olsson-Liljequist B, Zueva LP, et al. Epidemic strain of methicillin-resistant Staphylococcus aureus in hospitals of Saint-Petersburg. Zh Mikrobiol Epidemiol Immunobiol. 2010;5:24–29.

8. Online calculators for calculating statistical criteria. Available at: https://medstatistic.ru/calculators.html. Accessed: 12 October 2023 (In Russ).

9. Algburi A., Al-Hasani HM, Ismael TK, et al. Antimicrobial activity of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 against Staphylococcus aureus biofilms isolated from wound infection. Probiotics and Antimicrobial Proteins. 2021;13(1):125–134. doi: 10.1007/s12602-020-09673-4

10. Ahire J., Kashikar M., Lakshmi S., et al. Identification and characterization of antimicrobial peptide produced by indigenously isolated Bacillus paralicheniformis UBBLi30 strain. 3 Biotech. 2020;10(3):112–113. doi: 10.1007/s13205-020-2109-6

11. Vandini A, Temmerman R, Frabetti A, et al. Hard surface biocontrol in hospitals using microbial-based cleaning products. PLoS One. 2014;9(9):e108598. doi: 10.1371/journal.pone.0108598

12. Ilyakova A.V., Shestopalov N.V., Fedorova L.S., et al. Possibility of using spore-forming bacteria of the genus Bacillus in the production of disinfectants // Hygiene and sanitation. 2020; 99(5):436–442 (In Russ). doi: https://doi.org/10.33029/0016-9900-2020-99-5-436-442