Modern Approaches to the Methods of Creating of Phage Basis for Treatment-Prophylactic a Drug of Bacteriophages Pseudomonas aeruginosa

Therapeutic and prophylactic preparations of bacteriophages are considered as an effective modern means of antimicrobial therapy of Pseudomonas aeruginosa infection. Bacteriophages that are part  of the drugs must be certified for confirmation of their lytic and  genetic properties, which ensures the safety and effectiveness of the  use of drugs. In our study, biological and morphological  properties were studied for drug design, the spectrum of  antibacterial activity of bacteriophages isolated in various regions of  Russia was determined. The most important stage of the work was  research on ensuring genetic safety of bacteriophage preparations.  As a result, a composition of 6 strains of lytic bacteriophages of P.  aeruginosa (PaUfa №№ 1, 2, 3, 4, 6, 7) composed of the  morphology of the virions to the families Podoviridae and Myoviridae, the order of Caudovirales, phylogenetic genetic groups phiKMV- likevirus , N4-likevirus, PB1-likevirus. The composition has a wide  spectrum of antibacterial activity (90.7%) with respect to 818  collisional, epidemiologically significant hospital and clinical strains of P. aeruginosa bacteria, and also quite versatile for various remote  territories of Russia and CIS countries.

1. Kuznetsova M. V. The prevalence of the pathogen and the variety of nosological forms of Pseudomonas aeruginosa infection. Zdorov’ye Sem’i – 21 vek. [Family Health – the 21 Century]. 2014; 2: 84–112 (in Russian).

2. Lazareva A. V., Chebotar I. V., Kryzhanovskaya O. A., Chebotar V. I., Mayanskiy N. A. Pseudomonas aeruginosa: pathogenicity, pathogenesis and pathology. Klinicheskaya mikrobiologiya i antimikrobnaya himioterahiya. [Clinical Microbiology and Antimicrobial Chemotherapy]. 2015;tom 17 (3): 170–187 (in Russian).

3. Tikunova N. V., Voroshilova N. N., Genetic Characteristics and Range of Antibacterial Activity of the Bacteriophages, which are a part of manufactured serie of drugs – Pyobacteriophage Polyvalent Purifi ed. Epidemilogia i Vaccinoprofi lactica. [Epidemiology and Vaccinal Prevention]. 2016; 2 (87): 93 –100 (in Russian).

4. Dodova E. G.. Gorbunova E. A.. Apolikhina I. A. Post-antibiotic era: bacteriophages as a curative strategy. Meditsinskiy sovet. 2015; 11: 49–53.

5. Gabrielyan N. I., Gorskaya E. M., Spirina T. S., Prudnikova S. A., Romashkina L. Yu. Investigation of sensitivity of nosocomial microbial strains isolated from clinical transplant patients to antibiotics and phages. Vestnik Transplantologii i Iskusstvennykh Organov. [Russian Journal of Transplantology and Artifi cial Organs]. 2011; XIII (3): 26–32 (in Russian).

6. Salmina T.A.. Tsygipalo A.I.. Shkoda A.S. Experience in the use of a polyobenthic pyobacteriophage for treatment of purulent wounds with long-term and ineff ective treatment with antibacterial drugs. Trudny patsiyent. [Diffi cult Patient]. 2016; 10–11: 5–11 (in Russian).

7. General pharmacopoeial article «Bacteriophages, OFS.1.7.1.0002.15» State Pharmacopoeia of the Russian Federation. XIII edition. Volume II (in Russian).

8. Rules for conducting studies of biological medicinal products of the Eurasian Economic Union of 06.05.17g, decision No. 89 of 03.11.2016g. Available at: https://docs.eaeunion.org/docs/ru-ru/01411954/cncd_21112016_89 (www.eaeunion.org)

9. Bacteriophages. Mark H. Adams. Moskva: Izdatelstvo inostrannoy literatury; 1961.

10. Zhenhui Cao, Jiancheng Zhang, Yan D. Niu, Isolation and Characterization of a «phiKMV-Like» Bacteriophage and Its Therapeutic Eff ect on Mink Hemorrhagic рneumonia. PLoS One. Jan 23 2015; 10(1): e0116571.

11. Zuyev A. V. Letic activity of bacterial viruses. Moscow:Meditsina; 1969: 177 (in Russian).

12. Collection of techniques for the genetics of microorganisms. Moscow: Meditsina; 1970.

13. Katter E., Sulakvelidze A. Bacteriophages: Biology and Practical Applications. Moscow: Nauchnyy mir; 2012: 640.

14. Bacteriophage and its vectors. Ed.: Sambrook J., Russell D. Molecular Cloning. Cold Spring Harbour Laboratory Press. 2001.

15. Zelin Cui, Xiaokui Guo, Ke Dong, Safety assessment of Staphylococcus phages of the family Myoviridae based on complete genome sequences. PMC Journals. Jan 24. 2017; 7: 41259.