Antibacterial Activity of Benzydamine Hydrochloride against Clinical Isolates of Bacteria, isolated from people in Russia and Spain

Aim to study antibacterial activity of benzydamine hydrochloride, against clinic isolated of bacteria, located in hospitals in Russia and compare this data to results of similar trail of Spanish isolates.

Materials  and  method  for this study were used clinical isolates  of bacteria,  which are counted as typical agents  of infections in otorhinolaryngology and gynecology. Enterococcus spp., Escherichia coli, Klebsiella pneumoniae, Staphylococcus spp. and Streptococcus  spp.,  isolated from patients  in Russians  hospitals;  reference  strains  Escherichia coli, Staphylococcus  aureus and Streptococcus agalactiae, from ATCC collection; and also, Lactobacillus acidophilus strain, isolated from probiotic drug «Lactobacterin». Antibacterial activity of benzydamine hydrochloride was evaluated with serial dilution method in agar. For comparison of results we used data of Spanish study of benzydamine activity against clinical isolates and reference strains. The Spanish Study was completed in microbiological department of San-Pau hospital in Barcelona in 2001.

Results.  It was indicated that minimum inhibitory concentration (MIC) of benzydamine hydrochloride for clinical isolates of gram-negative and gram-positive  bacteria, isolated from Russian and Spanish hospitals is about the same level: for E. coli it was 640 – 1280  mg/l, for K. pneumoniae  – 512–1280  mg/l, for S. aureus  – 256–1280  mg/l, for S. agalactiae  – 320–1280  mg/l, for S. pyogenes – 256–640 mg/l, for E. faecalis – 512 mg/l, for E. faecium – 256 mg/l, for S. mitis – 640 mg/l, for S. epidermidis 320 – 1280 mg/l, for S. pneumoniae  –  40 mg/l, for S. viridans  –  40 mg/l. The same data was obtained by assessment of sensitivity to benzydamine hydrochloride reference-strains from ATCC collection: for E. coli MIC was 512 – 640 mg/l, for S. aureus – 512 – 640 mg/l, for S. agalactiae – 320 mg/l, for S. pneumoniae – 640 mg/l. Probiotic strain L. acidophilus was resistant to benzydamine hydrochloride activity with MIC = 20000 mg/l. Conclusion: It was indicated that antimicrobial activity of benzydamine hydrochloride against clinical strains, isolated from the patients of hospitals in Russia and Spain. Also, resistance of probiotic strain of lactobacteria was detected to this drug, which indicates the possibility of benzydamine hydrochloride application in clinical practice in otorhinolaryngology  and gynecology without risk of negative influence on normal lactobacterial flora.

1. Dunaevskij AM, Kirichenko IM. Klinicheskoe obosnovanie ispol’zovanija preparata miramistin v terapii infekcionno-vospalitel’nyh zabolevanij respiratornoj sistemy. Obzor literatury. Poliklinika 2013; 5–3: 6–12. (In Russ.)

2. Jurkin SA, Mihireva MM. Sovershenstvovanie mestnogo medikamentoznogo lechenija ostryh sinusitov. Rossijskaja rinologija. 2009; 17 (2): 32. (In Russ.)

3. Apolihina IA, Muslimova SZ. Bakterial’nyj vaginoz: chto novogo? Ginekologija. 2008; 10 (6): 36–37. (In Russ.)

4. Slavskij AN, Mejtel’ IJu. Bol’ v gorle: obosnovanie optimal’nogo vybora preparata. Medicinskij sovet 2016; 18: 128–132. (In Russ.)

5. Svistushkin VM, Nikiforova GN, Petrova EI. Bakterial’nye infekcii lor-organov: delikatnaja terapija. Medicinskij sovet. 2017; 8: 58–63. (In Russ.)

6. Kuchar E, Miśkiewicz K, Szenborn L, Kurpas D. Respiratory tract infections in children in primary healthcare in Poland.Adv Exp Med Biol. 2015; 835: 53–9.

7. Krasnova NA, Mironova NG. Bakterial’nyj vaginoz. Novyj metod vosstanovlenija biotopa vlagalishha. Vestnik Samarskogo universiteta. Estestvennonauchnaja serija. 2006;6-2 (46): 95–99. (In Russ.)

8. Serova OF, Zarochenceva NV, Men’shikova NS. Bakterial’nyj vaginoz: lechenie i profilaktika. Voprosy ginekologii, akusherstva i perinatologii. 2009;8(1):84-86. (In Russ.)

9. Kuznecova IV. Voprosy diagnostiki i lechenija infekcionnyh zabolevanij vlagalishha. Jeffektivnaja farmakoterapija. 2016; 14: 12–21. (In Russ.)

10. Ballesteros S, Ramón MF, Martínez-Arrieta R. Ingestions of benzydamine-containing vaginal preparations. Clin Toxicol (Phila). 2009; 47 (2): 145–149.

11. Fanaki NH, el-Nakeeb MA. Antimicrobial activity of benzydamine, a non-steroid anti-inflammatory agent. J Chemother. 1992; 4 (6): 347–352.

12. Prats G. Determination of Benzydamine antibacterial activity against clinical strains: an in vitro study. Servicio de Microbiologia, Hospital de Sant Pau, Barcelona, Spain.2001, study of Angelini ACRAF S.p.A. #8655U01.

13. Opredelenie chuvstvitel’nosti mikroorganizmov k antibakterial’nym preparatam, metodicheskie ukazanija MUK 4.2.1890-04, Klin Mikrobiol Antimikrob Himioter 2004; 6 (4): 306–359. (In Russ.)

14. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 13th Edition. CLSI supplement M2-A8. Wayne, PA. Clinical and Laboratory Standards Institute; 2004.

15. Svetoch EA., Tejmurazov MG, Abaimova AA, Fursova NK. Antibiotikorezistentnost’ i detekcija genov ustojchivosti k vankomicinu u kul’tur Enterococcus spp., vydelennyh ot ljudej i promyshlennoj pticy v Rossijskoj Federacii. Materialy XI s’ezda Vserossijskogo nauchno-prakticheskogo obshhestva jepidemiologov, mikrobiologov i parazitologov «Obespechenie jepidemiologicheskogo blagopoluchija: vyzovy i reshenija». M. 16-17 nojabrja 2017. P. 380. (In Russ.)

16. Prjamchuk SD, Fursova NK, Abaev IV, et al. Geneticheskie determinanty ustojchivosti k antibakterial’-nym sredstvam v nozokomial’nyh shtammah Escherichia coli, Klebsiella spp. i Enterobacter spp., vydelennyh v Rossii v 2003-2007 gg. Antibiotiki i himioterapija 2010; 55 (9–10): 3–10. (In Russ.)

17. Slukin PV, Astashkin EI, Ermolenko ZM, Fursova NK, Ershova MG. Geneticheskie determinanty antibiotikorezistntnosti i virulentnosti uropatogennyh Escherichia coli, vydelennyh v g. Jaroslavle v 2016–2017 gg. Materialy 1-go Rossijskogo mikrobiologicheskogo kongressa, 17-18 oktjabrja 2017. Pushhino-na-Oke; P. 124–125. (In Russ.)

18. Volozhantsev NV, Kislichkina AA, Lev AI, et al. Genome Sequences of Two NDM-1 Metallo-β-Lactamase-Producing Multidrug-Resistant Strains of Klebsiella pneumoniae with a High Degree of Similarity, One of Which Contains Prophage. Genome Announc. 2017; 5 (42): pii:e01173–17.

19. Skrjabin JuP, Pecherskih JeI, Korobova OV, Shishkova NA, Abaev IV. Identifikacija jepidemicheskih linij meticillin-rezistentnyh Staphylococcus aureus, vpervye vyjavlennyh v central’noj Rossii. Tezisy XIV Mezhdunarodnogo kongressa MAKMAH po antimikrobnoj himioterapii. Klinicheskaja mikrobiologija i antimikrobnaja terapija. 2012; 14 (2–S1): 20. (In Russ.)