The Role of Brucella abortus I-206 Thermoextracts in L- and S-form in Shaping the Immune Response in White Mice
V. I. Dubrovina,
T. P. Starovoitova,
S. A. Vityazeva,
N. L. Barannikova,
T. A. Ivanova,
G. B. Mukhturgin,
A. V. Gromova,
G. Yu. Sorkina,
S. V. Balakhonov https://doi.org/10.31631/2073-3046-2019-18-2-62-67
Abstract
Background. Brucellosis is an acute infectious-allergic zoonotic disease in which sick farm animals are the main source of infection for humans. Immunization of animals and people on the territory of Russia is carried out by live vaccines, which, having a high immunogenicity, can cause clinical, hematological and immune changes. Therefore, the work on the design of new vaccines that provide high protection and do not possess reactogenicity, agglutinogenic and sensitizing activity is the current direction of the study. Methodology. The study was performed on 72 outbred white mice immunized with inactivated B. abortus 19 ВA vaccine and thermal extracts (TE) of B. abortus I-206 in L- and S-form. Morphofunctional changes in the regional lymph node, spleen, and thymus of experimental animals were examined on days 3, 7, 14, and 21 of the time of immunization. Results.The influence of single introductions thermoextracts B. abortus I-206 in L-and S-form in comparison with inactivated vaccine B. abortus 19 ВА. the removal or the morphohistologic indices of immune organs of experimental animals. Found that thermoextracts have the ability to activate immune adjustment within the structures of the spleen and lymph nodes, without causing side effects, including allergic reactions. Conclusions. Preparations obtained from B. abortus in the L- and S-forms have the ability to activate immunological rearrangement in immunocompetent organs without causing any side effects. The results indicate the promise of further research in this direction.
About the Authors
V. I. Dubrovina
Irkutsk Antiplague Research Institute of Siberia and Far East.
Russian Federation
Russian Federation
Valentina I. Dubrovina – Dr. Sci. (Biol.), head of Pathophysiological laboratory.
78 Trilissera str. Irkutsk, Russia 664047.
T. P. Starovoitova
Irkutsk Antiplague Research Institute of Siberia and Far East.
Russian Federation
Russian Federation
Tat’yana P. Starovoitova – research officer of Pathophysiological laboratory.
78 Trilissera str. Irkutsk, Russia 664047.
S. A. Vityazeva
Irkutsk Antiplague Research Institute of Siberia and Far East.
Russian Federation
Russian Federation
Svetlana A. Vityazeva – Cand. Sci. (Med.), senior researcher of department of Plague microbiology.
78 Trilissera str. Irkutsk, Russia 664047.
N. L. Barannikova
Irkutsk Antiplague Research Institute of Siberia and Far East.
Russian Federation
Russian Federation
Natalia L. Barannikova – Cand. Sci. (Biol.), doctor-bacteriologist of department of Epidemiology.
78 Trilissera str. Irkutsk, Russia 664047.
T. A. Ivanova
Irkutsk Antiplague Research Institute of Siberia and Far East.
Russian Federation
Russian Federation
Tatyana A. Ivanova – head of the laboratory Experimental animals.
78 Trilissera str. Irkutsk, Russia 664047.
G. B. Mukhturgin
Irkutsk Antiplague Research Institute of Siberia and Far East.
Russian Federation
Russian Federation
Gennadiy B. Mukhturgin – junior researcher of laboratory of Experimental animals
78 Trilissera str. Irkutsk, Russia 664047.
A. V. Gromova
Irkutsk Antiplague Research Institute of Siberia and Far East.
Russian Federation
Russian Federation
Anastasia V. Gromova – Cand. Sci. (Vet.), research officer of Pathophysiological laboratory.
78 Trilissera str. Irkutsk, Russia 664047.
G. Yu. Sorkina
Irkutsk basic medical college.
Russian Federation
Galina Yu. Sorkina – teacher of the highest category.
Russian Federation
Galina Yu. Sorkina – teacher of the highest category.
S. V. Balakhonov
Irkutsk Antiplague Research Institute of Siberia and Far East.
Russian Federation
Russian Federation
Sergey V. Balakhonov – Dr. Sci. (Med.), professor, director.
78 Trilissera str. Irkutsk, Russia 664047.
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Review
For citations:
Dubrovina V.I., Starovoitova T.P., Vityazeva S.A., Barannikova N.L., Ivanova T.A., Mukhturgin G.B., Gromova A.V., Sorkina G.Yu., Balakhonov S.V. The Role of Brucella abortus I-206 Thermoextracts in L- and S-form in Shaping the Immune Response in White Mice. Epidemiology and Vaccinal Prevention. 2019;18(2):62-67. (In Russ.) https://doi.org/10.31631/2073-3046-2019-18-2-62-67
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