Assessment of the Level of Virus Neutralizing Antibodies to Enterovirus 71 in Children in Ekaterinburg (2010–2017)

Abstract

Aim of research. To analyze the results of the examination of children diagnosed with EVI, meningeal form in the period 2010–2017 and to assess the level of production of viral neutralizing antibodies to enterovirus 71 in children against the background of increasing epidemiological significance of this pathogen. Materials and methods. Nasopharyngeal flushes and faecal samples (2–3 samples) served as the material for virological research. In total for the period 2010–2017 into work were taken the clinical materials from 3558 people hospitalized in infectious diseases departments of hospitals of the city of Ekaterinburg. Enteroviruses were isolated using the method of infection of transplanted cell lines (RD, ner-2 and L20B). For serological studies (PH with reference strain EV71) were used paired serum samples of the same patients (n = 3336), taken at intervals of 14–21 days. Results and discussion. The percentage of positive results (the presence of specific antibodies in the titer 1:8 and above) among all the examined persons as a whole was 35.5%. Proportion of patients with of titers of specific antibodies to EV71 in the annual section ranged from 41.9% (2013) to 24.4% (2015). The maximum number among the examined patients (n = 2634) was children aged 1–14 years. The largest number of children with the viral neutralizing antibodies to EV-71 was in the group of patients 12 years and older (45.6%). In young children (3–7 years), the proportion of persons with serum antibodies was 28.9%. Conclusion. The results of our studies confirm the presence of type 71 Enterovirus circulation in the territory of Ekaterinburg. The maximum risk of possible infection is in the group of children in the age of 1–7 years.