Genetic Characteristics and Range of Antibacterial Activity of the Bacteriophages, which are a Part of Manufactured Serie of Drugs - Pyobacteriophage Polyvalent Purified

Pyobacteriophage polyvalent cleared (preparation) is active against 85.0 - 99.6% clinical strains of bacteria Proteus spp., Streptococcus spp., Klebsiella pneumoniae, Escherichia coli, Staphylococcus spp., and Pseudomonas aeruginosa. The preparation surpasses the majority of antibiotics in the width of a range of antibacterial activity and is comparable only with modern antibiotics of the last generations. Unlike the majority of antibiotics, the preparation doesn't cause toxic and allergic reactions, has no contraindications to application, including age, is allowed for use for newborns and children of early age that gives the chance to regard him as highly effective and safe medicine for antibacterial therapy. The results of metagenomic analysis of pyobacteriophage polyvalent cleared indicate that the product contains only lytic bacteriophages and does not contain lysogenic bacteriophages, that guarantees high concentration of lytic bacteriophages in a preparation high degree of his antibacterial and clinical efficiency and genetic safety of its clinical application.

бактерии, бактериофаги, антибиотики, антибактериальная активность, токсичность, побочные реакции, bacteria, bacteriophages, antibiotics, toxicity, antibacterial activity, side effects

1. Chernen'kaya T.V., Borisova L.A., Alexandrova I.V., Kosolapov D.A. Pathogens septic nosocomial infections in intensive care units ambulance hospital. Medical alphabet. Epidemiology and hygiene. 2013; (2): 30 – 33 (in Russian).

2. Shamayeva S.H., Mironov A.Yu., Matveev A.S., Potapov A.F., Golubev A.M. Microbiological monitoring of pathogens of nosocomial intra-abdominal infections and their antibiotic resistance in emergency surgical patients in the intensive care unit. Kursk scientific and practical bulletin «Chelovek I Ego Zdorovye». 2010; 4: 117 – 121 (in Russian).

3. Gabrielyan N. I., Gorskaya E.M., Spirina T.S., Prudnikova S. A., Romashkina L.Yu. Investigation of sensitivity of nosocomial microbial strains isolated from clinical transplant patients to antibiotics and phages. Herald of Transplantation and Artificial Organs. 2011; XIII (3): 26 – 32 (in Russian).

4. Grigoryevskaya Z.V., Petukhova I.N., Dmitriyeva N. V. The outbreak of the intrahospital infection caused by multiresistant (MDR) strains of K. pneumoniae. Siberian oncological magazine. 2014; 2 (62): 5 – 8 (in Russian).

5. Shaykhraziyeva N.D. Epidimiological monitoring of intrahospital infections in a surgical hospital. Kazan medical magazine, 2007; 88 (1): 87, 88 (in Russian).

6. Andreyeva S.V., Bakharev L.I., Valeeva D.M. Dynamics of antibiotic resistance to major pathogens of burn infections. Herald of the Chelyabinsk state university. 2013; 7 (2): 60 – 61 (in Russian).

7. Vorobyova O.N., Kamaleeva M.F., Denisenko L.I., Doshchitsina A.S., Dulepo S. A. Antibiotic resistance of pathogens of nosocomial infections in intensive care unit. The Russian medicobiological messenger of the academician I.P. Pavlov. 2009; 4: 30 – 36 (in Russian).

8. Swed S.I., Naumenko Z.S. , Rozova L.V., Shalaginova T.S., Klyushin N.M. Prevalence of drug resistance Proteus spp. in orthopedic and trauma hospital departments . Genius Ortopedii of 2004; 3: 20 – 24 (in Russion).

9. Lyamin A.V., Zhestov A.V. Antibiotic resistance of representatives of the family Enterobacteriaceae isolated from the mucous of the upper respiratory tract of blood donors. Pulmonology. Allergology. 2007; 4 (23): 22 – 24 (in Russian).

10. Boronina L.G., Blinova S.M., Lavrinenko E.B. Antibiotic resistance major pathogens of bronchopulmonary diseases in children in the Middle Urals in 2005 – 2009. Herald of the Ural medical academic science. 2010; 2: 7 – 11 (in Russian).

11. Dyachenko S.V., Zaporzhsky I.A., Slobodenyuk E.V. Antibiotic resistance of S. pneumoniae at patients with infections of respiratory tract. Far East Magazine of Infectious Pathology 2009; 14: 38 – 40 (in Russian).

12. Belopolskaya H.A., Sidorova I.S., Shakhgireeva L.S., Belopolsky A.A. Possibilities of fagovy therapy of a gynecologic infection. Difficult patient. 2014; 12 (8 – 9): 6 – 9 (in Russian).

13. Gayderov A.A. Studying the properties of Escherichia coli M-17 and Bacillus subtilis strains in 1719 on the model of experimental dysbiosis. Doctorate of med. sci. diss. Moscow; 2007 (in Russian).

14. Sultanov N.M., Voroshilova N.N, Aznabayev L.F., Arefyeva N.A. A comparative study of the antibacterial activity of the drug and immunotropic Piobakteriofag polyvalent purified and antibiotics for the treatment of chronic suppurative rhinosinusitis. Herald of the Ural medical academic science. 2006; 3 (1): 236 – 238 (in Russian).

15. Arefyeva N.A., Aznabayeva L.F., Voroshilova N.N., Sultanov N.M. Comparative study of the effect of treatments on the state of local immunity of the nasal mucosa of patients with chronic suppurative rhinosinusitis. Actual problems of science and education, II scientific conference with the international participation. Varadero (Cuba). Basic researches. 2007; 4: 51 – 53 (in Russian).

16. Arefyeva N. A., Sultanov N. M., Aznabayeva L.F., Voroshilova N.N. Rinotsitogramma of patients with a chronic suppurative rinosinusit at treatment by Piobakteriofag polyvalent cleared. Materials XVII of congress of otorhinolaryngologists of Russia. Ufa. 2006: 249 – 250 (in Russian).

17. Dautova Z.A., Fattakhov B.T., Voroshilova N.N., Serezhin I.N. The results of applying piobakteriofaga polyvalent purified liquid in traumatic keratitis and corneal ulcers. Materials of the All-Russian conference «Topical Issues of Development, Production and Application of Immunobiological and Pharmaceutical Preparations». Ufa. 2000: 58 – 61 (in Russian);

18. Khayrullin I.N., Pozdeev O.K., Shaymordanov R. Sh. The effectiveness of specific bacteriophages in the treatment and prevention of surgical postoperative infections. 2002; 4: 258 – 261 (in Russian).

19. Voroshilova N.N., Kazakova T.B., Bogovazova G.G., Alferova E.V., Usmanova S.S. Epidemiological and clinical efficacy of bacteriophage preparations in the treatment and prevention of infectious diseases. Creating and perspectives of medical immunobiological preparations. Materials of Scientific conference. Perm. 2008: 91 – 94 (in Russian).

20. Voroshilova N.N., Kazakova T.B., Bogovazova G.G., Usmanova S.S., Alferova E.V. Comparative study of the clinical and antibacterial efficacy of bacteriophages and antibiotics in the treatment of enteric diseases caused by opportunistic bacteria. Materials 12 of the International Slavyano-Baltiysky forum «Sankt-St. Petersburg-Gastro-2010». Gastroenterology of St. Petersburg. 2010; 2 (3): 18 , 19 (in Russian).

21. Pushkarev A.M., Voroshilova N.N., Korzhavin G.V. Etiotropic treatment of nosocomial infections in postoperative patients in urological hospital. Materials III All-Russian scientific-practical conference «New Technologies in Surgery». Ufa. 1998: 258 (in Russian).

22. Lakhno V.M., Bordunovsky V.I. Application a fagotherapy in surgical practice. Herald of surgery. 2001; 160 (1): 122 – 125 (in Russian).

23. Bessmertnaya N.G., Chizhova G.V., Vladimirova N.Yu., Kogut E.P. et al. Efficiency of application a piobakteriofag for pregnant women with pyelonephritis. Modern methods of diagnostics and treatment in obstetrics and gynecology. Collection of scientific works. Saratov. 1999: 35 – 36 (in Russian).

24. Zarivchatsky M.F., Mugatarov I.N., Shvetsova Yu.A. The use preparations of bacteriophage for the treatment of inflammatory diseases of soft tissues. Creating and perspectives of medical immunobiological drugs: materials of the All-Russian scientific and practical conference. Perm. Item, 2008: 104 – 106 (in Russian).

25. Zakharova Yu.A., Belokrylov N.M. Clinical case of treating a patient with osteomyelitis, caused by multidrug-resistant Pseudomonas aeruginosa and Staphylococcus aureus using the method of phage therapy, Creating and perspectives of medical immunobiological drugs: Materials of the All-Russian scientific and practical conference. Perm: 2008: 96, 97 (in Russian).

26. Kisina V.I., Perepanova T.S., Zabirov K.I., Bednova V.N., Vasil'ev М.М., Darbeeva О.S. et al. Phage therapy of inflammatory urogenital diseases at women. Herald of dermatology and venereology. 1996; 5: 45 – 48 (in Russian).

27. Verbeken G., Pirnay J.-P., Lavigne R., Jennes S., De Vos D., Casteels M., Huys I. Call for a dedicated european legal framework for bacteriophage therapy. archivum immunologiae et therapiae experimentalis. 2014; 62 (2): 117 – 129.

28. Bacteriophage λ and its vectors. In: Molecular Cloning, Eds. Sambrook J, Russell D. Cold spring harbour laboratory press. New York: 2001: 187 – 303.