Molecular Epidemiology of Tuberculosis Infection in Omsk Region

Relevance. In the Omsk region in 2005 - 2014 tended to decrease morbidity of tuberculosis infection. However, the dynamics of tuberculosis with multidrug resistance was characterized by a moderate upward trend from 29.6 to 38.3 per 100 thousand population (P < 0.05). Goal. Characteristics of the epidemic process of tuberculosis in the Omsk region for 10 years, molecular genetic features of leading genotypes of M. tuberculosis, circulating in the area to evaluate and select the most effective measures to prevent the spread of multidrug-resistant tuberculosis. Materials and methods. Studied with MIRU-VNTR-typing M. tuberculosis isolates from patients with active pulmonary tuberculosis (n = 100). All patients were inhabitants of Omsk region and treated in health organizations of TB in 2013 - 2014. Results. 36 genetic types have been identified in the result of typing. The composition of the 10 clusters of various sizes included 74 isolates. For genetic family Beijing treated 77.0% of isolates clustered (OR = 25.7, CI 6.87 * 96.17). Among the unique isolates of M. tuberculosis was dominated by unidentified genotypes, non-family Beijing (OR = 0.11, 95% CI 0,04 * 0,30), as well as rare genotypes Uganda I, Haarlem, S. Frequency clustering in this study was 64%, indicating that a significant proportion of cases of tuberculosis associated with active Mycobacterium tuberculosis transmission. Conclusions. The data obtained are of interest for the evaluation and selection of the most effective measures to prevent the spread of strains of multi-drug resistance in the Omsk region.

эпидемиология, распространенность, MIRU-VNTR-типирование, кластеры, генотипы, генотип Beijing, epidemiology, prevalence, MIRU-VNTR-typing, clusters, genotypes, genotype Beijing

1. Pasechnik O.A., Stasenko V.L., Bloh A.I. Epidemic Manifestations of Tuberculosis in Regions of Siberia with Various Prevalence of HIV Infection. Epidemiologiya i Vakcinoprofilaktika [Epidemiology and Vaccinal Prevention]. 2015; 5 (84): 31 – 35 (in Russian).

2. Pasechnik O.A., Astafurova E.D., Bokareva R.V., Kortusova L.N. Characteristics of the Spectrum of drug resistance of Mycobacterium tuberculosis among new cases. Sovremennye problemy nauki i obrazovaniya [Modern problems of science and education]. 2014; 6: 1024 (in Russian).

3. Mokrousov I.V. Methodological approaches to Mycobacterium tuberculosis genotyping for Evolutionary and Epidemiological research. Infeksiya i immunitet. [Infection and Immunity]. 2012; 3: 603 – 614 (in Russian).

4. Ribeiro F.K., Pan W., Bertolde A.,Vinhas S. A., Peres R.L., Riley L. Genotypic and spatial analysis of Mycobacterium tuberculosis transmission in a highincidence urban setting. Clin. Infect. Dis. 2015; 61 (5): 758-66. DOI: 10,1093/CID/civ365.

5. Narvskaya O.V., Mokrousov I.V.,Vyazovaya A.A., Lyalina L.V., Shulgina M.V., MyasnikovaE.B. et al. Causing agent molecular-genetic analysis in TB epidemiological surveillance. Medicinskij al›yans [Medical alliance]. 2014; 1:75 – 78 (in Russian).

6. Embden J.D. van, Cave M.D., Crawford J.T., Dale J.W., Eisenach K.D., Giequel B. et al. Strain identification of Mycobacterium tuberculosis by DNA Fingerprinting: Recommendations for a Standartized Methodology. J. Clin. Microbiol.1993; 31 (2): 406 – 409.

7. Supply P., Allix C., Lesjean S., Cardoso-Oelemann M., Rüsch-Gerdes S., Willery E. et al. Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. J. Clin. Microbiol. 2006; 44 (12): 4498 – 4510.

8. Dymova M. A., Alkhovik O. I., Cherednichenko A. G., Khrapov E. A., Petrenko T. I., Filipenko M. L. Genotyping of isolates of Mycobacterium tuberculosis,characterized by extensively drug resistance.Vestnik NSU. Vestnik NGU. Seriya: Biologiya, klinicheskayamedicina [Herald of NSU. Series: biology, clinical medicine. 2013; 1: 110 – 117 (in Russian).

9. Nava-Aguilera E., Andersson N., Harris E. Mitchell S., Hamel C., Shea B. et al. Risk factors associated with recent transmission of tuberculosis: systematic review and meta-analysis. Int. J. Tuberc. Lung Dis. 2009; 13: 17 – 26.

10. Munch Z., Van Lill S.W., Booysen C.N., Zietsman H.L., Enarson D.A., Beyers N. Tuberculosis transmission patterns in a higt-incidence area: a spatial analysis. Int. J. Tuberc. Lung Dis.2003;7: 271-7.

11. Kontsevaya I.S., Nikolayevskyy V.V. The role of Mycobacterium tuberculosis Beijing lineage in tuberculosis epidemiology in Russian Federation. Biomika [Biomics]. 2014; 1: 13 – 23 (in Russian).

12. Lyashenko A.A. Genetic variants of Mycobacterium tuberculosis and their epidemiological importance. ScienceRise. 2015; 3/4 (8): 13 – 18 (in Russian).

13. Ogarkov O.B., Zhdanova S.N., Mokrousov I.V., Vinokurova M.K., Alekseeva G.I., Baranova Y.A. et al. Molecular epidemiological features of M. tuberculosis family S in Sakha (Yakutia). Sibirskij Medicinskij Zurnal [Siberian Medical Journal]. 2014; (6):109 – 111 (in Russian).

14. Fok A., Numata Y., Schultzer M., Fitzgerald M.J. Risk factors for clustering of tuberculosis cases: a systematic review of population-based molecular epidemiology studies. Int. J. Tuberc. lung Dis. 2008. 12 (5): 480 – 492.

15. Andreevskaya S.N., Chernousova L.N., Smirnova T.G., Larionova E.E., KuzminA.V. Transmission of Mycobacterium tuberculosis strains, due to migration processes in the Russian Federation. Tuberkulez i bolezni legkih [Tuberculosis and Lung Disease]. 2006; 1: 29 – 35 (in Russian).