Technology for Producing Hyperimmune Agglutinating Sera against Salmonella using Various Immunization Schemes and Studying the Biochemical and Immunological Parameters of the Sera

The spread of typhoid and paratyphoid fevers across all countries highlights their significance as a major public health concern (with Paratyphoid B also being relevant for veterinary medicine). The use of agglutinating diagnostic sera enables the serological identification of Salmonella bacteria through agglutination reactions. Purpose – to produce hyperimmune agglutinating sera against Salmonella using various immunization schemes and to study the biochemical and immunological parameters of the obtained serum samples. Materials and Methods. The following Salmonella strains were used for immunization (in the production of hyperimmune antisera): S. typhi 002140/4446, S. typhi 003788/18, S. typhi 003909/135, S. typhi 003901/418, S. typhimurium 004453/11, S. enteritidis 000571/867, S. paratyphi B 001150/34, S. anatum 001022/885, S. paratyphi A 000652/217. These strains were obtained from the National Collection of Pathogenic Microorganisms (Groups III-IV of human infections) at the Republican Specialized Scientific and Practical Medical Center of Epidemiology, Microbiology, Infectious and Parasitic Diseases (RSSPMCEIPD) of the Ministry of Health of the Republic of Uzbekistan. Corpuscular antigens of inactivated Salmonella strains were used for immunization. The hyperimmune sera were studied using bacteriological, biochemical, serological, and statistical methods. Results. Experimental research led to the creation of a bank of 72 diagnostic serum samples for Salmonella detection. The study of polyvalent diagnostic sera showed an increase in total protein, globulin, IgA, and IgG levels after the first, second, and third immunizations (on days 7, 14, and 21). Conclusion. Given that the levels of total protein, albumin, globulin, and IgG in the experimental animals peaked on day 28 of immunization, sera with high specific activity against various Salmonella strains can be obtained after four weeks.

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