Targeted Sequencing in Infectious Disease Diagnostics: Current State and Future Prospects
https://doi.org/10.31631/2073-3046-2026-25-1-111-120
Abstract
Relevance. Targeted sequencing is becoming an increasingly important tool in the diagnosis of infectious diseases in response to the limitations of conventional diagnostic methods and the high cost and complexity of metagenomic sequencing. Aims. To summarize current approaches for targeted next-generation sequencing (tNGS) in infectious disease diagnostics, to highlight its advantages and limitations in comparison with culture-based and PCR-based methods as well as metagenomic sequencing, and to discuss prospects for its implementation in routine clinical practice and genomic surveillance systems. Conclusion. Targeted sequencing has been shown to substantially improve the sensitivity and specificity of pathogen detection while maintaining turnaround time and cost comparable to conventional diagnostic methods. In addition, it enables accurate pathogen typing and the simultaneous generation of data suitable for genomic surveillance and antimicrobial resistance monitoring. Key technological and organizational barriers are discussed, along with future directions in the development of targeted panels and bioinformatic solutions that may establish tNGS as one of the core tools of infectious disease diagnostics in the coming years.
About the Authors
M. I. Nadtoka
FBSI Central Research Institute of Epidemiology of Rospotrebnadzor
Russian Federation
Russian Federation
Maksim Ig. Nadtoka – Research associate
+7 (916)080-97-50
G. V. Roev
FBSI Central Research Institute of Epidemiology of Rospotrebnadzor;
Moscow Institute of Physics and Technology (National Research University)
Russian Federation
Russian Federation
German V. Roev – Bioinformatician; Postgraduate
+7 (925) 440-35-50
K. F. Khafizov
FBSI Central Research Institute of Epidemiology of Rospotrebnadzor
Russian Federation
Russian Federation
Kamil F. Khafizov – Head of the laboratory
+7 (917) 597-20-85
V. G. Akimkin
FBSI Central Research Institute of Epidemiology of Rospotrebnadzor
Russian Federation
Russian Federation
Vasily G. Akimkin – Director
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Review
For citations:
Nadtoka M.I., Roev G.V., Khafizov K.F., Akimkin V.G. Targeted Sequencing in Infectious Disease Diagnostics: Current State and Future Prospects. Epidemiology and Vaccinal Prevention. 2026;25(1):111-120. (In Russ.) https://doi.org/10.31631/2073-3046-2026-25-1-111-120
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