Microbiological Evidence of Etiology «Ixodes Tick-Borne Borreliosis without Erythema Migrans» - Infection Caused by Borrelia miyamotoi
https://doi.org/10.31631/2073-3046-2017-16-1-29-35
Abstract
Background. The spirochete Borrelia miyamotoi belongs to the group of borrelia causing tick-borne relapsing fevers. It is transmitted by the same tick species as Borrelia burgdorferi sensu lato and is widespread in temperate climate zones of Eurasia and North America. Previously we have shown by PCR that clinical cases presented as systemic febrile illness and diagnosed usually as «Ixodes tick-borne borreliosis without erythema migrans» were caused by B. miyamotoi. According to recent estimation in Russia there are several thousands human cases of B. miyamotoi infection annually. The investigation of this emerging human pathogen was hampered because the attempts to culture this bacterium directly from human blood failed. Results and conclusion: We here describe protocol - avoiding the inhibitory effects of anticoagulants on B. miyamotoi growth - enabling successful B. miyamotoi isolation from blood of Russian patients.
About the Authors
A. E. Platonov
Federal State Institution of Science «Central Research Institute of Epidemiology» Federal service on customers1 rights protection and human well-being surveillance
Russian Federation
Russian Federation
J. . Koetsveld
Academic Medical Center, University of Amsterdam
Russian Federation
Russian Federation
N. M. Kolyasnikova
Federal State Institution of Science «Central Research Institute of Epidemiology» Federal service on customers rights protection and human well-being surveillance
Russian Federation
Russian Federation
D. S. Sarksyan
Federal State Institution of Science «Central Research Institute of Epidemiology» Federal service on customers rights protection and human well-being surveillance; State Educational Institution of Higher Professional Training «Izhevsk State Medical Academy» of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
M. G. Toporkova
Ltd «Medical association «Novaya Bolnitsa»
Russian Federation
Russian Federation
G. A. Shipulin
Federal State Institution of Science «Central Research Institute of Epidemiology» Federal service on customers rights protection and human well-being surveillance
Russian Federation
Russian Federation
J. W. Hovius
Academic Medical Center, University of Amsterdam
Russian Federation
Russian Federation
References
1. Platonov A.E., Karan L.S., Kolyasnikova N.M., Makhneva N.A., Toporkova M.G., Maleev V.V., et al. Humans infected with relapsing fever spirochete Borrelia miyamotoi, Russia. Emerg. Infect. Dis. 2011; 17 (10): 1816 – 1823.
2. Platonov A.E., Maleev V.V., Karan L.S. Relapsing borrelioses fevers: forgotten and new ones. Terapevtichesky Arkhiv. [Therapeutic archives]. 2010; 82 (11): 74 – 80 (In Russian).
3. Wagemakers A., Staarink P.J., Sprong H., Hovius J.W. Borrelia miyamotoi: a widespread tick-borne relapsing fever spirochete. Trends Parasitol. 2015; 31 (6): 260 – 269.
4. Sarksyan D.S., Platonov A.E., Karan L.S., Malinin I.E., Khalitova L.I., Shakhov V.I., et al. Clinical presentation of «new» tick-borne borreliosis caused by Borrelia miyamotoi. Terapevtichesky Arkhiv. [Therapeutic archives]. 2012; 84(11): 34 – 41 (In Russian).
5. Platonov A.E., Sarksyan D.S., Maleev V.V. The application of decision trees for constructing an algorithm for the differential diagnosis of zoonotic infections. Terapevtichesky Arkhiv. [Therapeutic archives]. 2013; 85 (11): 21 – 26 (In Russian).
6. Platonov A.E., Sarksyan D.S., Karan L.S., Shipulin G.A., Gordygina E.V., Malinin O.V., et al. The blood coagulation system and microcirculatory disorders in ixodid tick-borne borreliosis caused by Borrelia miyamotoi. Terapevtichesky Arkhiv. [Therapeutic archives]. 2015; 87(11): 26 – 32 (In Russian).
7. Sarksyan D.S., Maleev V.V., Platonov A.E., Platonova O.V., Karan L.S. Relapsing (recurrent) disease caused by Borrelia miyamotoi. Terapevtichesky Arkhiv. [Therapeutic archives]. 2015; 87(11): 18 – 25 (In Russian).
8. Molloy P.J., Telford S.R., Chowdri H.R., Lepore T.J., Gugliotta J.L., Weeks K.E., et al. Borrelia miyamotoi disease in the northeastern United States: a case series. Ann. Intern. Med. 2015; 163 (2): 91 – 98.
9. Fukunaga M., Okada K., Nakao M., Konishi T., Sato Y. Phylogenetic analysis of Borrelia species based on flagellin gene sequences and its application for molecular typing of Lyme disease borreliae. Int. J. Syst. Bacteriol. 1996; 46 (4): 898 – 905.
10. Scoles G.A., Papero M., Beati L., Fish D. A relapsing fever group spirochete transmitted by Ixodes scapularis ticks. Vector Borne Zoonotic Dis. 2001; 1 (1): 21 – 34.
11. Wagemakers A., Oei A., Fikrig M.M., Miellet W.R., Hovius J.W. The relapsing fever spirochete Borrelia miyamotoi is cultivable in a modified Kelly-Pettenkofer medium, and is resistant to human complement. Parasit. Vectors. 2014; 7: 418.
12. Margos G., Stockmeier S., Hizo-Teufel C., Hepner S., Fish D., Dautel H., et al. Long-term in vitro cultivation of Borrelia miyamotoi. Ticks Tick Borne Dis. 2015; 6 (2): 181 – 184.
13. Barbour A.G., Tessier S.L., Stoenner H.G. Variable major proteins of Borrellia hermsii. J. Exp. Med. 1982; 156 (5): 1312 – 1324.
Review
For citations:
Platonov A.E., Koetsveld J., Kolyasnikova N.M., Sarksyan D.S., Toporkova M.G., Shipulin G.A., Hovius J.W. Microbiological Evidence of Etiology «Ixodes Tick-Borne Borreliosis without Erythema Migrans» - Infection Caused by Borrelia miyamotoi. Epidemiology and Vaccinal Prevention. 2017;16(1):29-35. (In Russ.) https://doi.org/10.31631/2073-3046-2017-16-1-29-35
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