The paper examines the experience of employee surveys foodservice, food contact for the presence of pathogens of acute intestinal infections during the XXII Olympic Winter Games and XI Paralympic Winter Games of 2014 in Sochi. It also shows the data on the work organization, taking into account its features and of laboratory test results. In the conclusion it is considered the effectiveness of the measures.

The organization of laboratory environmental monitoring in the pre-Olympic period and during the XXII Olympic winter games and XI Paralympic winter games 2014. The results of radiation monitoring during the construction of sports and infrastructure facilities Sochi. Study analyzed the procedure for compliance with hygienic standards and additional indicators of drinking water, food, food raw material and other objects.

We have presented a comparative analysis of morbidity and mortality in the tick-borne encephalitis (TBE) in the south of the Russian Far East in the period 1980 - 2014. In the period from 2008 to 2014 using the enzyme-linked immunoassay (ELISA) was examined 10,599 copies ticks or leukocyte fraction of blood (n = 5561) of people who indicate to the fact of tick bites. Blood samples and ticks positive in ELISA additionally examined by PCR and virus isolation. Under diagnosis of TBE cases in the 1980s resulted in lower figures morbidity and mortality artificially excessive to 39% (average 28%). In the 1990s there was a sharp rise in the incidence of TBE (median 15.3% mortality). In the 2000s there was a trend to a decrease in the number of cases and deaths (average case fatality rate of 6.3 -10.5%). The incidence rate for 35-year follow-up period ranged from 0.85 to 9.88 per 100 thousand population, mortality was 14.8 ± 0.7%. The degree of danger of the epidemic on TBE synchronously reflected in the rate of antigen detection of TBE virus in humans and ticks attached themselves. In the year’s high morbidity and mortality (in 2009 and 2010) antigen detection in ticks and in human blood reached a maximum. Isolated from the blood of these patients three TBE virus strains indicated that only a complete virus capable of replicating determines the variety clinical forms of the infection.

Hepatitis C virus (HCV), which was identified in 1989 - 1990. Later it was included in new genus Hepacivirus of the family Flaviviridae. Due the genetic heterogeneity of HCV, viral isolates was decided to classificate on genotypes and sybtypes in accordance with the sequence of nucleotides in a certain area of the genome. It was discover that the virus infect only human and chimpanzee. Modern molecular epidemiological data, obtained after 2000, indicate monocentric origin of HCV from Africa, most likely from the central part. Probably, in Africa the conditions for feeling HCV-like virus from unknown non-primate mammal to human were formed. Recently HCV-like viruses were found in dogs, horses, bars, and rodents. The most common hypothesis of the HCV origin base on unknown virus, which is belong to genus Hepacivirus and does not infect human-like primate. The virus is included into group of nonprimate hepacivirus (NPHV). In last centure in our country viral intergenotype recombinant RF_2k/1b was appeared. In modern time HCV diversity can appeared as serology poor displayed forms of the virus. Drug-resistant variants of HCV can accumulate and disseminate again the background of antiviral therapy.

Aim of study: to give a comparative epidemiological characteristic of hepatitis C in Mongolia and Irkutsk region taking into account races, INF λ3 gene polymorphism and INF λ4 gene polymorphismin studied population. Epidemiological evidence of hepatitis C in Mongolia, in contrast to the Irkutsk region, characterized higher prevalence, domination of seropositive persons in age older than 50 years, domination in the circulation of the 1st virus genotype. In the population of Mongolia was revealed high frequency some nucleotide INF λ3 and INF λ4 gene polymorphism. These genetic markers are more favorable for natural history of hepatitis C. It explains the high rate of spontaneous hepatitis C virus clearance among people of Asian race.

Different species of small mammals (SM) (539 total), cached in Voronezh region, were examined for antigen and DNA of Francisella tularensis. The basic species of SM involved in circulation of F. tularensis were revealed. The features of the biotopic distribution of infected SM on the territory of the natural foci were shown. The diversity of species SM ensures long operation and epizootic activity of the natural foci of tularemia.

Analysis of dynamics and trends in parenteral viral hepatitides and tuberculosis morbidity rates in Moscow for 1999 - 2013. Analysis of dynamics and trends in parenteral viral hepatitides and tuberculosis morbidity rates in Moscow in 1999 - 2013 based on the statistical data of Moscow regional information collection of federal state-financed healthcare institution Moscow city centre of hygiene and epidemiology. In aggregate, 1389 cards and the data of 24 forms were processed. The tuberculosis morbidity rate of the population of the city of Moscow in 1999 - 2009 exhibited tendency towards increasing and in 2009 - 2013 there was a tendency towards decreasing of the morbidity rate. As distinguished from the aggregate population situation, the tuberculosis morbidity rate among children was increasing steadily between 2003 and 2013. There was a massive children’s morbidity rate peak in 2011 - 2012 which may be caused by both increase of the number of patients and by improvement of diagnostics techniques. While the morbidity rate of virulent forms of hepatitis has decreased, the increase of the morbidity rate of chronic forms of hepatitis B and hepatitis C among the aggregate population of Moscow has been registered. The change in the age distribution of patients getting down with all forms of viral hepatitis B and C towards older patients has been observed. Men get virulent and chronic forms of hepatitis B and C 1.3 - 3.9 times more than women. The analysis of the transmission routes structure of hepatitis B and C has enabled us to establish the main risk factors contributing to transmission of these infectious diseases, namely casual sexual intercourse, drug addiction and insufficient supervision of the safety of procedures involving skin penetration in medical institutions. The main transmission route is sexual (heterosexual) transmission. Conclusion. The special aspects of the present epidemiological situation of hepatitis B and C and tuberculosis in the city of Moscow have been ascertained in the article.

The results of a cohort study of 177 newborns, from birth until they were 18 months old, are presented. The 87 of those children had lacked impact of maternal HIV infection, and 90 children were not exposed to HIV prenatally. Antisocial factors were observed among the newborns exposed to a prenatal HIV infection which had a negative impact upon them throughout infancy. The social deviance of their family members with a HIV infection was 10.5 times higher than the standard risk of a neonatal abstinence syndrome, 4 times higher than a standard risk of growth retardation during fetal development, and 3.8 times higher than a standard risk of postnatal development disorders. Undiagnosed infants who were exposed to a HIV-infection constitute a medico-social risk group requiring scrupulous and extended medical assistance.

An assessment of the current toxocariasis situation in the Russian Federation was made. Several data sources have been examined for this purpose - statistical materials on the toxocariasis incidence of the Russian population in 2008 - 2012, the Regional Services on Customers’ Rights Protection and Human Well-Being Surveillance State reports concerning the administrative territories in 2010 -2012 about the sanitary-epidemiological wellbeing of the population, the results of their own research and the published data. Nowadays, in different regions of the Russian Federation, there is considerable variation in toxocariasis incidence values. On a number of areas located in similar climatic conditions and landscape-geographical zones recorded both low and high levels of morbidity, the most pronounced among children. Antibodies to toxocara detected in different climatic zones of the population, the proportion of seropositive, according seroepidemiological studies is 5.4 - 33.6% of adults and 11.8 - 35.2% among children. It is concluded that the incidence of toxocariasis significantly higher reported rates of statistical observation. The need to develop a guidance document, reflecting the diagnostic value of clinical and laboratory signs of toxocariasis and epidemiological evidence for screening for toxocariasis threatened populations.

It was developed a new kit of reagents «Line-Blot TORCH-profile» to define in the format of linear immunoblotting antibodies to main agents TORCH group (kit N1 - for IgG and kit N 2 - for IgM). In the study of 141 certified controls industrial production showed 100% sensitivity and specificity of studies with a new kit. The study in ELISA and linear immunoblotting with developed set and its counterpart foreign production of clinical samples submitted from persons held diagnostic testing, made it possible to calculate the clinical informativity of both studied methods and corresponding sets of reagents according to GOST R 53022.3-2008. This has led to the recommendation set No.1 (IgG) as an alternative test to the use instead of several ELISA kits for determining monospecific antibodies to each of the major pathogens of TORCH group, and the set No.2 (IgM) - to determine active phase of infection or timing of infection.

The evaluation of acute and chronic toxicity and local effect of the inactivated influenza vaccine Ultriks in animal models (guinea pigs, mice BALB/c) was performed. No vaccine-related toxic effect after a single intraperitoneal injection, multiple intramuscular injections, and no local irritant effect after a single intramuscular injection were reported in experimental animals.

Etiology of respiratory virus infections among 1699 hospitalized patients (HP) was determined by PCR during the period of increased influenza activity in 2012 - 2013 season. The rate of accidence of influenza virus in dependence of gender, age, social and demographic factors and previous vaccination was analysed. Young children dominated in the age structure of HP, while the elderly were hospitalized very rarely. According to results obtained rate of influenza detection in HP was significantly higher among adults in comparison with children (63,5 and 30,7% of investigated patients, correspondingly). Respiratory syncytial virus and rhinoviruses were detected the most regularly (8.7 and 3.1%, correspondingly) in children, parainfluenza and adenoviruses were registered rarely (1.4 and 2.1%, correspondingly). Rate of detection of coronaviruses and bocavirus was low and varied in the range 0.3 - 0.6%. Indicated above ARI agents among hospitalized adults were detected rarely (0 - 1.5%) with exception of RSV which was detected among elderly (75 - 84 years) in 5.9% cases. No metapneumovirus cases were detected among HP in indicated period. Although males dominated (58%) among HP influenza cases, regardless of the type/subtype, were registered more frequently among girls in comparison with the boys of the same age groups. Influenza cases were registered more frequenly as well among smoking than in not smoking patients. Young children dominated in the age structure of HP, while the elderly were hospitalized very rarely.

The present study of antipertussis immunity stress and level in young and school children who was vaccinated ADTP vaccine showed that on the average 28.3% of them were seronegative. The lowest parts of seronegative children were detected in the age group of infants under 12 months of age (12.3%) and in that of 15 - 17 years old teenagers (12.1%). The maximum percent of seronegative children were detected in the age group of 6 - 8 years - 38.8%. Despite the nonsignificant increase of this indicator, compared to the previous age group, it is advisable to supplement serological monitoring of indicator group 6- 7 years. The researchers did not discover the reliable correlation between the pertussis incidence in different age groups and proportion of seronegative children in those groups. The obtained results suggest that there is an occult circulation of pertussis causing agent. They also showed that it is necessary to revise the indicator age groups for serologic monitoring of antipertussis immunity.

Recommendations highlighted features vaccination of children with anemia. It is shown how the immunization scheme may be changed according to the pathology and the age of the child.