The academician V. D. Belyakov - one of the outstanding scientists-epidemiologists who have exerted fruitful impact on development of domestic epidemiological science and preventive medicine in general. In article the main stages of scientific activity of the scientist are considered: development of the theory of internal regulation of parasitic systems; creation of a paradigm of modern epidemiology as diagnostic and preventive discipline of medical science and practical health care, etc. Are shown possible ways of further development of the ideas of V. D. Belyakov and their introduction to scientific, student and preventive teaching.

The fundamental theoretical and methodological shortcomings of modern organisation and implementation of epidemiological surveillance of natural focal infections are considered. The conclusion is made about the need to prepare a realistic programme of observations of state change in natural focal parasitic systems and contacts of the population with them. Its main goal is to obtain ecologo-epidemiological and socio-demographic data needed for short and medium term forecasting of the epidemiological manifestations of natural foci.

The aim of the study was to obtain the complex characteristics of tick-borne encephalitis virus (TBEV) of European subtype circulating in Western and Eastern Siberia. Using full-genome sequencing approach it was demonstrated that TBEV strains of European subtype isolated in Siberia are genetically similar to the strains from European part of its habitat range, and with the representatives from South Korea. It was confirmed that the homology of TBEV strains of European subtype isolated in different parts of the virus habitat area from Scandinavian countries in the west to the eastern borders of the area (South Korea) is much higher than the homology level of TBEV strains of Far Eastern and Siberian subtypes. The Siberian population of TBEV of European subtype is presented with two groups of strains called as Eastern Siberian and Western Siberian variants, which differ in the combinations of amino acid substitutions in all proteins except NS2B protein. It is found that TBEV strains of European subtype from Siberia possess high neurovirulence, but some of them, like strains from Europe, demonstrate low invasiveness. It is shown that TBEV strains of European subtype have good adaptive capacity, and therefore, can easily adapt to the circulation in various biocenoses in the territory of different landscape-geographical zones. It was found that the circulation of TBEV of European subtype is fixed in Siberia territory for over 40 years. It was emphasized that in spite of circulation of TBEV of European subtype in the significantly different areas by climatic conditions, topography, landscape, habitat characteristics it possesses a high degree of genome stability.

The results of the study with modern molecular genetic research methods the circulation in the Kyrgyzstan territory avian flu virus in waterfowl and humans. Tested 3032 samples from 106 species of wild aquatic birds, of which 46 samples detected the flu virus, the proportion of positive samples was 1.5% (95% CI = 1.1 1.9). In 17 species of birds able to detect flu viruses. Avian influenza virus is more likely to appear among the following species of wild aquatic birds: black-headed gull, mallard, snipe, river terns, coots, common teal. Influenza A(H5N1) was found only in three samples from three birds: caspian gull, black-headed gull and mallard. Among people with clinical symptoms of acute respiratory infection and flui the laboratory testing virus A(H5N1) is not detected. The level of flu and acute respiratory viral infection of the population in areas located near lakes and wintering wetland birds was similar to that in other regions of the country.

Systematics and nomenclature of representatives Rickettsiales order has always been based on a limited number of available phenotypic characteristics, due to their obligate intracellular localization. Intensive use of «molecular» technology has led to the description of a large number of new species of this taxonomic group, as well as to the reclassification of some of its representatives. Another symbol of the era of «molecular» technology is the emergence of species, genera and families with candidate status («Candidatus») due to the inability of cultivation and studying of biological properties that is required for a valid description of the new species. It should be a legitimate solution to this problem, based on the development and application of new high technologies. This review summarizes recent data on the taxonomy, nomenclature and evolution of representatives order Rickettsiales, new technologies, approaches and methods for the study of certain groups of prokaryotes. Published materials were obtained in the analysis of foreign and Russian scientific publications, as well as on the basis of its own published data. The paper contains a new understanding of the classification within the genus Rickettsia.

We consider the safety of domestic products combined (DTP, Bubo Kok) containing whole-cell pertussis vaccine. DTP and Bubo Kok safety vaccines meet WHO requirements, EF and more rigid in some cases, the requirements of Russian regulatory documents. Thus, in assessing the safety of specific pertussis vaccine and complete neutralization of diphtheria and tetanus toxins, are administered to laboratory animals much larger doses of test products in comparison with the requirements of the WHO and the EP. In contrast to the WHO and the EP in the domestic requirements have stringent requirements for quantitative content in the non-adsorbed toxoids vaccine. Compared with foreign counterparts, domestic vaccines are significantly less anti-genetic load, that is responsible for their low reactivity, at the same time they possess the desired immunogenic activity.

The article presents the results of research quality of drugs for the prevention of smallpox during the period from 2007 to 2015 years, where the reference standard uses industry standard of activity, specificity (identification) and necrotic activity of smallpox vaccine, on indicators: «Specific activity», «The antigenic activity» and «Specific safety». Described research methods, presented results and statistical analysis of the results. The article also provided the results of the regular re-certification of reference standard sample activity, specificity (identification) and necrotic activity smallpox vaccine OOO 42-28-113-12 (ОСО), held in 2015. Branch reference preparation for the determination of activity, specificity and necrotic activity of the smallpox vaccine was re-certified with the « specific activity» assigned value of 4,9 ± 2,7 х 10⁸ pfu/ml. Other certified characteristics were also confirmed. The criteria «Specific activity» and «Specificity (identification)» were determined using chorioallantoic membrane of 12-days-old chicken embryos and compared against the First International reference preparation of the smallpox vaccine. The re-certification of the branch reference preparation for the determination of activity, specificity (identification) and necrotic activity of the smallpox vaccine (42-28-113-12) confirmed the possibility of its further use. The shelf-life before the next re-certification is 3 years.

The current state of the problem of Brucella interactions with the host in the review is discussesed. Provides information about the mechanisms of persistence of Brucella in the host organism due to their ability long remain in the cells immunoregulatory system. Data on the differences of Brucella in smooth and rough forms on invasive ability are presented. The key role of the macrophage in Brucella infection is shown. Promising areas of research in the field of immunobiology of brucellosis are indicated.

The immunogenic and protective activities of recombinant IgA1 serine protease obtained on the base of the genome DNA of N. meningitidis serogroup B strain H44/76 were studied. A several recombinant proteins of different molecular weights that are based on the full-length primary structure of the enzyme, taking into account the distribution of B- and T-epitopes, also were studied. In experiments on laboratory animals it was shown that a number of tested preparations demonstrate the immunogenic and protective activity to protect mice from lethal challenge with virulent strains of meningococcus serogroups A, B and C, thereby exhibiting polyvaccine properties. The protective role of antibodies against the IgA1 protease was shown when mice were infected by meningococccus serogroup B. The increase in antibodies to the meningococcal IgA1 protease into the blood of rabbits infected with different serotypes of pneumococci has been detected, indicating potential ability of the meningococcal IgA1 protease to generate protection against microbes the virulence of which is caused by IgA1protease.

Detection of post-vaccination complications with the subsequent investigation of causes and measures to prevent their occurrence increases the perception of the immunization society and improves health care. This is primarily increases the immunization coverage, which leads to lower morbidity. The purpose of this article is to offer for discussion a new algorithm for the registration of diseases postvaccination period, based on a synthesis of the existing national system and modern international approaches.